Journal
RSC ADVANCES
Volume 9, Issue 61, Pages 35797-35802Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9ra07028a
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Funding
- DFG [INST 187/621-1, INST 187/686-1, CO 1328/2-1]
- China Scholarship Council [2016 08310143]
- Leibniz Universitat Hannover
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Ectopic expression of BC1 which encodes a putative pathway specific transcription factor from the ACE1 biosynthetic gene cluster of the rice pathogen Pyricularia oryzae Guy11 did not lead to the production of ACE1-related compounds. However the known compound hinnulin A was formed. A putative partial gene cluster potentially involved in the biosynthesis of hinnulin A and DHN melanin was validated by RT-PCR and a possible biosynthetic pathway is proposed. Ectopic expression of pyiR which encodes a pathway specific transcription factor from the pyrichalasin H biosynthetic gene cluster in Magnaporthe grisea NI980 led to the apparent up-regulation of the pyi cluster and a 3-fold increase in pyrichalasin production under standard fermentation conditions, but did not lead to the formation of new compounds.
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