Journal
JOURNAL OF MEDICAL ENTOMOLOGY
Volume 54, Issue 6, Pages 1786-1789Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/jme/tjx170
Keywords
high-throughput DNA sequencing; PCR; blood meal identification; disease vector; triatominae
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Funding
- UGA Office of the Vice President for Research, Secretaria Nacional de Ciencia Tecnologia e Inovacion (SENACYT) [COL11-043]
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1156707] Funding Source: National Science Foundation
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Accurate blood meal identification is critical to understand hematophagous vector-host relationships. This study describes a customizable Next-Generation Sequencing (NGS) approach to identify blood meals from Rhodnius pallescens (Hemiptera: Reduviidae) triatomines using multiple barcoded primers and existing software to pick operational taxonomic units and match sequences for blood meal identification. We precisely identified all positive control samples using this method and further examined 74 wild-caught R. pallescens samples. With this novel blood meal identification method, we detected 13 vertebrate species in the blood meals, as well as single and multiple blood meals in individual bugs. Our results demonstrate the reliability and descriptive uses of our method.
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