Journal
CLINICAL & TRANSLATIONAL IMMUNOLOGY
Volume 8, Issue 11, Pages -Publisher
WILEY
DOI: 10.1002/cti2.1094
Keywords
anti-CTLA-4; anti-PD-1; tissue-specific microenvironment; trimAb; tumor microenvironment
Categories
Funding
- Cancer Australia [1100199]
- Peter MacCallum Cancer Center Foundation
- National Health and Medical Research Council (NHMRC) of Australia [1132373]
- National Breast Cancer Foundation (NBCF) of Australia [IIRS-18-064]
- Susan G Komen Breast Cancer Foundation [16376637]
- Australian Postgraduate Award
- NHMRC
- NBCF
- Cancer Council Victoria
- Research Foundation Flanders [1S32316N]
- National Health and Medical Research Council of Australia [1132373] Funding Source: NHMRC
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Objectives Investigation of variable response rates to cancer immunotherapies has exposed the immunosuppressive tumor microenvironment (TME) as a limiting factor of therapeutic efficacy. A determinant of TME composition is the tumor location, and clinical data have revealed associations between certain metastatic sites and reduced responses. Preclinical models to study tissue-specific TMEs have eliminated genetic heterogeneity, but have investigated models with limited clinical relevance. Methods We investigated the TMEs of tumors at clinically relevant sites of metastasis (liver and lungs) and their impact on alpha PD-1/alpha CTLA4 and trimAb (alpha DR5, alpha 4-1BB, alpha CD40) therapy responses in the 67NR mouse breast cancer and Renca mouse kidney cancer models. Results Tumors grown in the lungs were resistant to both therapies whereas the same tumor lines growing in the mammary fat pad (MFP), liver or subcutaneously could be completely eradicated, despite greater tumor burden. Assessment of tumor cells and drug delivery in 67NR lung or MFP tumors revealed no differences and prompted investigation into the immune TME. Lung tumors had a more immunosuppressive TME with increased myeloid-derived suppressor cell infiltration, decreased T cell infiltration and activation, and decreased NK cell activation. Depletion of various immune cell subsets indicated an equivalent role for NK cells and CD8(+) T cells in lung tumour control. Thus, targeting T cells with alpha PD-1/alpha CTLA4 or trimAb was not sufficient to elicit a robust antitumor response in lung tumors. Conclusion Taken together, these data demonstrate that tissue-specific TMEs influence immunotherapy responses and highlight the importance in defining tissue-specific response patterns in patients.
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