Journal
LAB ON A CHIP
Volume 19, Issue 24, Pages 4064-4070Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9lc00656g
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Funding
- NSF [DMR-1708729]
- Harvard MRSEC [DMR-1420570]
- National Science Foundation under NSF [ECS-1541959]
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We report an additive-free method to lyse bacteria and extract nucleic acids and protein using a traveling surface acoustic wave (TSAW) coupled to a microfluidic device. We characterize the effects of the TSAW on E. coli by measuring the viability of cells exposed to the sound waves and find that about 90% are dead. In addition, we measure the protein and nucleic acids released from the cells and show that we recover about 20% of the total material. The lysis method should work for all types of bacteria. These results demonstrate the feasibility of using TSAW to lyse bacteria in a manner that is independent of the type of bacteria.
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