Supplemented powdered coconut water (ACP-406®) promotes growth of goat secondary follicles and oocyte meiotic resumption

The objective of this study was to test the efficiency of powdered coconut water (ACP-406 (R)) base-medium without or with the addition of supplements on in vitro culture of isolated goat secondary follicles. Follicles were cultured for 18 days in alpha-MEM or in ACP-406 (R), both without supplements (referred to as alpha-MEM and ACP, respectively), or both supplemented with BSA, insulin, transferrin, selenium, glutamine, hypoxanthine, and ascorbic acid (referred to as alpha-MEM+ and ACP(+)). Follicular morphology, antrum formation, follicular and oocyte diameter, levels of glutathione (GSH), and chromatin configuration after in vitro maturation were evaluated. At the end of culture, ACP-406 (R) base-medium (without or with supplements) showed a higher (P < 0.05) percentage of normal follicles than alpha-MEM (without or with supplements). Antrum formation was similar among alpha-MEM+, ACP and ACP(+), and significantly higher than alpha-MEM without supplements. The follicular diameter was greater in ACP(+) than alpha-MEM, and similar to other treatments. Moreover, fully and daily grown rates were higher (P < 0.05) in ACP-406 (R) base-medium (without or with supplements) than alpha-MEM (without or with supplements). Levels of GSH were similar between ACP(+) and alpha-MEM+ treatments. Both ACP(+) and alpha-MEM+ allowed meiotic resumption without a significant difference between the two groups. In conclusion, supplemented ACP-406 (R) base-medium maintained follicular survival and promoted the development as well as meiotic resumption of isolated goat secondary follicles cultured in vitro for 18 days.