Journal
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
Volume 427, Issue -, Pages 71-80Publisher
ELSEVIER
DOI: 10.1016/j.jmmm.2016.11.010
Keywords
Magnetic nanoparticles; Cellular uptake; Magnet; Culture; Homogeneity
Funding
- Ministry of Science and Technology, Taiwan [NSC 100-2120-M-182-001-]
- National Health Research Institute, Taiwan [NHRI-EX9909937EI]
- Healthy Aging Research Program at Chang Gung University [EMRPD1E1651]
- Chang Gung Memorial Hospital [BMRP432]
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Magnetic force may greatly enhance uptake of magnetic nanoparticles (MNPs) by cultured cells; however, the effects of non-uniformity of magnetic field/magnetic gradient on MNP internalization in culture has not been elucidated. Cellular uptake of polyacrylic acid coated-MNP by LN229 cells was measured with cylindrical NdFeB magnets arranged in a staggered pattern. The magnetic field generated by placing a magnet underneath (H-field) elicited a homogenous distribution of MNPs on the cells in culture; whereas the field without magnet underneath (L-field) resulted in MNP distribution along the edge of the wells. Cell-associated MNP (MNPcell) appeared to be magnetic field-and concentration-dependent. In H-field, MNPcell reached plateau within one hour of exposure to MNP with only one-min application of the magnetic force in the beginning of incubation; continuous presence of the magnet for 2 h did not further increase MNPcell, suggesting that magnetic force-induced uptake may be primarily contributed to enhanced MNP sedimentation. Although MNP distribution was much inhomogeneous in L-field, averaged MNPcell in the L-field may reach as high as 80% of that in H-field during 1-6 h incubation, suggesting high capacity of MNP internalization. In addition, no significant difference was observed in MNPcell analyzed by flow cytometry with the application of H-field of staggered plate vs. filled magnet plate. Therefore, biological variation may dominate MNP internalization even under relatively uniformed magnetic field; whereas non-uniformed magnetic field may serve as a model for tumor targeting with MNPs in vivo.
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