Antimicrobial activities and absorption properties of disazo dyes containing imidazole and pyrazole moieties

The newly synthesized thirteen disazo dyes containing imidazole and pyrazole cycles (3a-3m) were carried out by diazotization reaction of 5-amino-4-arylazo-3-methyl-1H-pyrazoles (2a-2m) and coupling reaction with 1-methyl imidazole. Structures of synthesized azo dyes were characterized by UV-Vis, FT-IR, H-1-NMR, elemental analysis and their solvatochromic properties were studied in DMSO, DMF, methanol, acetic acid and chloroform. In addition, the antimicrobial activities of dyes against twelve pathogenic bacteria were examined in detail and all dyes showed antimicrobial activity against different bacterial strains.