Journal
JOURNAL OF LIPID RESEARCH
Volume 58, Issue 12, Pages 2275-2288Publisher
ELSEVIER
DOI: 10.1194/jlr.M079012
Keywords
fatty acyls; glycerolipids; lipids; phospholipids; quality control; quantitation; sphingolipids; Standard Reference Material 1950; sterols; National Institute of Standards and Technology
Categories
Funding
- National Institutes of Health [Lipid Metabolites and Pathways Strategy (LIPID MAPS)] [U54 GM069338, R01 GM20501-41, P30 DK064391]
- Research Center for Transomics Medicine, Kyushu University
- ALCA
- AMED-CREST
- Japan Science and Technology Agency [JPMJCR1395]
- Natural Sciences and Engineering Research Council of Canada
- Swedish Research Council [2015-4870]
- National Center for Advancing Translational Sciences Grant [UL1 TR000040]
- Foundation for the National Institutes of Health [5P01CA120964, 5P30CA006516, P01 HL034300]
- National Institute of General Medical Sciences [PO1 GM095467]
- National Center for Research Resources [S10RR027926]
- National Institutes of Health [P20 HL113452, U24 DK097154]
- Southeast Center for Integrated Metabolomics (SECIM) via National Institutes of Health [U24 DK097209]
- Austrian Science Fund (FWF) [P26148-N19]
- Southampton Centre for Biomedical Research (SCBR)/NIHR Southampton Respiratory Biomedical Research Unit
- Metabolomics Innovation Centre (TMIC) through the Genome Innovations Network (GIN) from Genome Canada
- Genome Alberta
- Genome British Columbia for operations [205MET, 7203]
- Canadian Institutes of Health Research [FDN143309]
- Canadian Foundation for Innovation [CFI 12156]
- Kansas Lipidomics Research Center (KLRC)
- National Science Foundation [MCB 1413036, MCB 0920663, DBI 0521587, DBI 1228622]
- Kansas INBRE (Foundation for the National Institutes of Health from the INBRE Program of the National Center for Research Resources)) [P20 RR16475]
- NSF EPSCoR [EPS-0236913]
- Kansas Technology Enterprise Corporation
- Kansas State University
- Swedish Heart-Lung Foundation [HLF 20140469, HLF 20150640]
- Stable Isotope and Metabolomics Core Facility of the Diabetes Research and Training Center (DRTC) of the Albert Einstein College of Medicine
- National Institutes of Health/NCI [P60DK020541]
- Biomarkers Core Laboratory, Irving Institute for Clinical and Translational Research
- Columbia University Medical Center
- National Center for Advancing Translational Sciences, National Institutes of Health [UL1 TL001873]
- National University of Singapore through Life Sciences Institute
- Yong Loo Lin School of Medicine Department of Biochemistry
- Department of Biological Sciences
- Singapore National Research Foundation [NRFI2015-05]
- Leading Edge Endowment Fund (University of Victoria)
- Segal McGill Chair in Molecular Oncology at McGill University (Montreal, Quebec, Canada)
- Warren Y. Soper Charitable Trust
- Alvin Segal Family Foundation (Montreal, Quebec, Canada)
- [215MET]
- [MC3T]
- Steno Diabetes Center Copenhagen (SDCC) [SDCC 3.A Complications] Funding Source: researchfish
- Grants-in-Aid for Scientific Research [15K06557] Funding Source: KAKEN
- Austrian Science Fund (FWF) [P26148] Funding Source: Austrian Science Fund (FWF)
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As the lipidomics field continues to advance, self-evaluation within the community is critical. Here, we performed an interlaboratory comparison exercise for lipidomics using Standard Reference Material (SRM) 1950-Metabolites in Frozen Human Plasma, a commercially available reference material. The interlaboratory study comprised 31 diverse laboratories, with each laboratory using a different lipidomics workflow. A total of 1,527 unique lipids were measured across all laboratories and consensus location estimates and associated uncertainties were determined for 339 of these lipids measured at the sum composition level by five or more participating laboratories. These evaluated lipids detected in SRM 1950 serve as community-wide benchmarks for intra-and interlaboratory quality control and method validation. These analyses were performed using nonstandardized laboratory-independent workflows. The consensus locations were also compared with a previous examination of SRM 1950 by the LIPID MAPS consortium.jlr While the central theme of the interlaboratory study was to provide values to help harmonize lipids, lipid mediators, and precursor measurements across the community, it was also initiated to stimulate a discussion regarding areas in need of improvement.
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