Journal
JOURNAL OF INVESTIGATIVE DERMATOLOGY
Volume 137, Issue 11, Pages 2354-2361Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jid.2017.03.042
Keywords
-
Categories
Funding
- National Research Foundation of Korea - Korean Government [2014R1A5A2009242, 2012M3A9B6055416, 2016R1A2B4008513, 2015R1D1A3A01016229]
- KRIBB Research Initiative Program [KGM4251723]
- National Research Foundation of Korea [2015R1D1A3A01016229] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
House dust mites have been implicated in the etiology and exacerbation of atopic dermatitis. Diverse factors contribute to house dust mite allergenicity through the activation of innate immunity. We investigated whether Dermatophagoides farinae extract (DFE) allergens mediate innate immune activation through specific toll-like receptors (TLRs) in epidermal keratinocytes, a DFE-induced murine atopic dermatitis model, and human atopic dermatitis lesions. DFE activated the expression of TLR1, TLR6, IL-25, and IL-33 in human primary keratinocytes and HaCaT cells. Knockdown of TLR6 inhibited DFE-induced upregulation of IL-25 or IL-33. In addition, the suppression of TLR1 inhibited the release of IL-33. DFE induced the expression of IL-25 and IL-33 by upregulation of IL-1 receptor-associated kinase 1, transforming growth factor-beta activated kinase-1, IkB kinase, and NFkB pathways. Tlr6(-/-) mice did not show DFE-induced upregulation of IL-25 and IL-33. Furthermore, DFE-induced upregulation of IL-25 was not induced in Tlr1(-/-) mice. We also identified upregulated mRNA and protein expression of TLR1, TLR6, IL-25, and IL-33 in human atopic dermatitis skin lesions with high house dust mite sensitization. We found that DFE-induced activation of TLR1 and TLR6 may cause polarization toward a T helper type 2 immune response via the release of IL-25 and IL-33.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available