Journal
JOURNAL OF INVERTEBRATE PATHOLOGY
Volume 144, Issue -, Pages 11-17Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2017.01.006
Keywords
Spodoptera littoralis; Bacillus amyloliquefaciens AG biosurfactant; Vip3Aa16; Toxicity; Histology; Putative binding receptors
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Funding
- Tunisian Ministry of Higher Education and Scientific Research
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Spodoptera littoralis, one of the most serious and destructive agricultural pests in the world, is very susceptible to Vip3 toxin. In order to develop a new efficient bioinsecticide and to prevent the development of resistance by the target pest, insecticidal activity of biosurfactant produced by Bacillus amyloliquefaciens AG1 was evaluated against S. littoralis. Bioassays revealed the susceptibility of the first instar larvae of this pest to AG1 biosurfactant with an LC50 of 245 ng/cm(2). Moreover, the histopathology examination of the larval midgut treated by AG1 biosurfactant showed vacuolization, necrosis and disintegration of the basement membrane. Binding experiments revealed that the AG1 biosurfactant recognized three putative receptors located in the brush border membrane vesicles of S. littoralis with sizes of 91, 72 and 64 kDa. Competition assays using biotinylated metabolites indicated that AG1 biosurfactant and Vip3Aa16 toxin did not compete for the same S. littoralis receptors. When combined, AG1 biosurfactant and Vip3Aa16 showed an additive effect against S. littoralis larvae. These findings suggested that B. amyloliquefaciens AGI biosurfactant could be a promising biocontrol agent to eradicate S. littoralis and to prevent resistance development by this pest. (C) 2017 Elsevier Inc. All rights reserved.
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