Journal
ANTIBIOTICS-BASEL
Volume 8, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/antibiotics8040236
Keywords
Maowei Sea; mangrove; actinobacteria; diversity; ESKAPE; dereplication; GNPS; quinomycins
Categories
Funding
- CAMS Innovation Fund for Medical Sciences [CAMS2017-I2M-BR-08]
- PUMC Doctoral Innovation Fund Project [2018-1007-16]
- NSFC (National Natural Science Foundation of China) [81611530716, 81460537]
- RFBR (Russian Foundation for Basic Research) [17-53-53130]
- Russian Foundation for Basic Research [18-34-20055]
- Guangxi Key Laboratory Cultivation Base Construction Project [17-259-74]
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Rediscovery of known antibiotics from actinobacteria, especially Streptomyces, has become a bottleneck issue. Nowadays, more specific identification and dereplication could be acquired by a combination of modern analytic techniques with various databases. In this study, 261 actinobacterial strains were isolated from 8 mangrove soil samples by culture-dependent method. A total of 83 strains were selected to evaluate antibacterial activities and mechanisms by disc diffusion method and a unique double fluorescent protein reporter system (pDualrep2), respectively. Thirty-two strains exhibited antagonistic activity against at least one of the ESKAPE pathogens. Four Streptomyces strains (B475, B486, B353, and B98) showed strong inhibitory activity against Gram-positive bacteria and induced DNA damage SOS response. One Micromonospora strain (B704) exhibited inhibitory activity against several pathogens and induced attenuation-based translational inhibitors reporter. Seven members of quinoxaline-type antibiotics including quinomycin A, quinomycin monosulfoxide, and other five putative new analogues were found from the culture broth of strain B475 by a combination of anti-MRSA guide, HPTLC, HPLC-UV, and UPLC-UV-HRESIMS/MS analysis, Chemspider searching, and MS/MS-based molecular networking analysis. In conclusion, this study not only demonstrated that mangrove is a rich source of actinobacteria with the potentially new antibiotics but showed rapid dereplication of known antibiotics in the early stage can improve efficiency for the discovery of new antibiotics.
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