Journal
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
Volume 59, Issue 2, Pages 118-133Publisher
WILEY
DOI: 10.1111/jipb.12515
Keywords
CRISPR; Cas9; CBF; cold tolerance; COR; single CBF-regulated genes
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Funding
- National Natural Science Foundation of China [91331201]
- Ministry of Agriculture of China [2014ZX08009-001]
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The transcription factors CBF1/2/3 are reported to play a dominant role in the cold responsive network of Arabidopsis by directly regulating the expression levels of cold responsive (COR) genes. In this study, we obtained CRISPR/Cas9-mediated loss-of-function mutants of cbf1 approximate to 3. Over 3,000 COR genes identified by RNA-seq analysis showed a slight but significant change in their expression levels in the mutants compared to the wild-type plants after being treated at 4 degrees C for 12h. The C-repeat (CRT) motif (5-CCGAC-3) was enriched in promoters of genes that were up-regulated by CBF2 and CBF3 but not in promoters of genes up-regulated by CBF1. These data suggest that CBF2 and CBF3 play a more important role in directing the cold response by regulating different sets of downstream COR genes. More than 2/3 of COR genes were co-regulated by two or three CBFs and were involved mainly in cellular signal transduction and metabolic processes; less than 1/3 of the genes were regulated by one CBF, and those genes up-regulated were enriched in cold-related abiotic stress responses. Our results indicate that CBFs play an important role in the trade-off between cold tolerance and plant growth through the precise regulation of COR genes in the complicated transcriptional network.
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