Journal
JOURNAL OF INORGANIC BIOCHEMISTRY
Volume 166, Issue -, Pages 190-198Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2016.09.001
Keywords
Metal ions; Cosolutes; G-quadruplex; Hoogsteen base pair; Topology
Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
- MEXT-Supported Program for the Strategic Research Foundation at Private Universities, Japan [16H02283, 15K17888, 24245033]
- JSPS
- MESS under the Japan-Slovenia Research Cooperative Program
- Hirao Taro Foundation of KONAN GAKUEN for Academic Research
- Okazaki Kazuo Foundation of KONAN GAKUEN for Advanced Scientific Research
- Chubei Itoh Foundation
- Grants-in-Aid for Scientific Research [15K17888, 16H02283] Funding Source: KAKEN
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Topologies of G-quadruplexes depend on oligonucleotide sequences and on environmental factors, and the diversity of G-quadruplex topologies complicates investigation of functions of these nucleic acid structures. To investigate how metal ions and cosolutes regulate topologies of G-quadruplexes, we stabilized the antiparallel conformation by insertion of 2'-deoxyxanthosine and 8-oxo-2'-deoxyguanosine into selected positions of an oligonucleotide. Thermodynamic analyses of the oligonucleotide revealed that Na+ stabilized the antiparallel G-quadruplex, whereas K+ destabilized this topology. This result suggests that metal ions selectively stabilize G-quadruplex topologies with cavities between G-quartet planes of certain sizes. In the presence of KCl in 20 wff, poly(ethylene glycol) with average molecular weight of 200, the antiparallel basket-type G-quadruplex conformation was not stabilized compared with the dilute condition. In the presence of NaCl, the cosolute did stabilize the G-quadruplex with respect to the dilute condition. The presented data show that metal ions and cosolutes regulate topologies of G-quadruplexes through mechanisms that depend on sizes of metal ion cavities and hydration states. (C) 2016 Elsevier Inc. All rights reserved.
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