Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume 44, Issue 8, Pages 1127-1135Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-017-1940-1
Keywords
5-Aminolevulinic acid; The C4 pathway; Metabolic engineering; Coenzyme A; HemB
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Funding
- National Natural Science Foundation of China [31670092]
- Natural Science Foundation of Jiangsu Province [BK20141107]
- Key Technologies R&D Program of Jiangsu Province, China [BE2014607]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT_15R26]
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5-Aminolevulinic acid (ALA), the first committed intermediate for natural biosynthesis of tetrapyrrole compounds, has recently drawn intensive attention due to its broad potential applications. In this study, we describe the construction of recombinant Escherichia coli strains for ALA production from glucose via the C4 pathway. The hemA gene from Rhodobacter capsulatus was optimally overexpressed using a ribosome binding site engineering strategy, which enhanced ALA production substantially from 20 to 689 mg/L. Following optimization of biosynthesis pathways towards coenzyme A and precursor (glycine and succinyl-CoA), and downregulation of hemB expression, the production of ALA was further increased to 2.81 g/L in batch-fermentation.
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