Journal
GENOME BIOLOGY
Volume 20, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s13059-019-1897-7
Keywords
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Funding
- National Science Foundation of China [31801097, NSFC31430025, 31870893]
- Beijing Municipal Science & Technology Commission [Z181100001318009]
- Beijing Advanced Innovation Center for Genomics at Peking University
- Peking-Tsinghua Center for Life Sciences
- National Major Science & Technology Project for Control and Prevention of Major Infectious Diseases in China [2018ZX10301401]
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Identification of functional elements for a protein of interest is important for achieving a mechanistic understanding. However, it remains cumbersome to assess each and every amino acid of a given protein in relevance to its functional significance. Here, we report a strategy, PArsing fragmented DNA Sequences from CRISPR Tiling MUtagenesis Screening (PASTMUS), which provides a streamlined workflow and a bioinformatics pipeline to identify critical amino acids of proteins in their native biological contexts. Using this approach, we map six proteins-three bacterial toxin receptors and three cancer drug targets, and acquire their corresponding functional maps at amino acid resolution.
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