Journal
JOURNAL OF CANCER
Volume 10, Issue 26, Pages 6649-6659Publisher
IVYSPRING INT PUBL
DOI: 10.7150/jca.29213
Keywords
HCC; lncRNA MALAT1; miR-125a-3p; FOXM1
Categories
Funding
- National Natural Science Foundation of China [81402579]
- Key Program of research and development Foundation of Shandong [2017GSF18179]
- Source Innovation Foundation of Qingdao [18-2-2-79-jch]
- Clinical Medicine + X of Qingdao University [CMX201729]
Ask authors/readers for more resources
Background: Hepatocellular carcinoma (HCC) is a prominent cancer type, with long non-coding RNAs (lncRNAs) being known to be relevant to its progression. We therefore investigated how a particular lncRNA known as the metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) was associated with HCC. Methods: Quantitative reverse transcriptase PCR (qPCR) was used to assess expression of MALAT1, Forkhead Box M1 (FOXM1) and miR-125a-3p in HCC tissue samples. How MALAT1 regulates HCC proliferation and metastasis was assessed through appropriate assays. FOXM1 was identified as a miR-125a-3p target using luciferase assays, and how MALAT1/miR-125a-3p alter FOXM1 expression was explored via qPCR and Western blotting. Results: HCC tissues exhibited MALAT1 upregulation. miR-125a-3p targeted FOXM1 and could be regulated by MALAT1. MALAT1 knockdown disrupted proliferation and invasion, whereas miR-125a-3p knockdown partially reversed this phenotype. Conclusions: These results indicate that MALAT1 modulates FOXM1 expression via being a miR-125a-3p sponge, thus promoting HCC progression.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available