Journal
DEVELOPMENTAL CELL
Volume 34, Issue 1, Pages 73-84Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2015.05.012
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Funding
- NSF [1024973, 1244146]
- NIH [GM108829]
- Cancer Research UK [C3/A11431]
- Medical Research Council [G1001696]
- FEBS Fellowship
- Medical Research Council [G1001696] Funding Source: researchfish
- MRC [G1001696] Funding Source: UKRI
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1244146] Funding Source: National Science Foundation
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Centromeres are essential chromosomal structures that mediate accurate chromosome segregation during cell division. Centromeres are specified epigenetically by the heritable incorporation of the centromeric histone H3 variant CENP-A. While many of the primary factors that mediate centromeric deposition of CENP-A are known, the chromatin and DNA requirements of this process have remained elusive. Here, we uncover a role for transcription in Drosophila CENP-A deposition. Using an inducible ectopic centromere system that uncouples CENP-A deposition from endogenous centromere function and cell-cycle progression, we demonstrate that CENP-A assembly by its loading factor, CAL1, requires RNAPII-mediated transcription of the underlying DNA. This transcription depends on the CAL1 binding partner FACT, but not on CENP-A incorporation. Our work establishes RNAPII passage as a key step in chaperone-mediated CENP-A chromatin establishment and propagation.
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