Journal
DEVELOPMENTAL CELL
Volume 35, Issue 3, Pages 383-394Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2015.10.010
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Funding
- NIH [R01GM103785, R01HD073104, P40OD010997]
- Charles A. King Trust Postdoctoral Fellowship Program
- Burroughs-Wellcome Fund
- Edward Mallinckrodt, Jr. Foundation grant
- Bank of America, N.A.
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A biochemical explanation of development from the fertilized egg to the adult requires an understanding of the proteins and RNAs expressed over time during embryogenesis. We present a comprehensive characterization of protein and mRNA dynamics across early development in Xenopus. Surprisingly, we find that most protein levels change little and duplicated genes are expressed similarly. While the correlation between protein and mRNA levels is poor, a mass action kinetics model parameterized using protein synthesis and degradation rates regresses protein dynamics to RNA dynamics, corrected for initial protein concentration. This study provides detailed data for absolute levels of similar to 10,000 proteins and similar to 28,000 transcripts via a convenient web portal, a rich resource for developmental biologists. It underscores the lasting impact of maternal dowry, finds surprisingly few cases where degradation alone drives a change in protein level, and highlights the importance of transcription in shaping the dynamics of the embryonic proteome.
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