Journal
DEVELOPMENTAL BIOLOGY
Volume 400, Issue 1, Pages 43-58Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2015.01.014
Keywords
Spermatogenesis; Common marmoset; Spermatogonia; Spermatocyte; Gonocyte; Germ cell
Categories
Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT)
- Ministry of Health, Labour and Welfare
- Japan Society for the Promotion of Science (JSPS)
- National Institute of Biomedical Innovation
- MEXT
- Funding Program for World-Leading Innovative R&D in Science and Technology (FIRST)
- Keio University
- Leave a Nest Grant Life Technologies Japan Award
- Interuniversity Bio-Backup Project for Basic Biology
- Otsuka Toshimi Foundation
- Grants-in-Aid for Scientific Research [25860222] Funding Source: KAKEN
Ask authors/readers for more resources
Mammalian spermatogenesis has been investigated extensively in rodents and a strictly controlled developmental process has been defined at cellular and molecular levels. In comparison, primate spermatogenesis has been far less well characterized. However, important differences between primate and rodent spermatogenesis are emerging so it is not always accurate to extrapolate findings in rodents to primate systems. Here, we performed an extensive immunofluorescence study of spermatogenesis in neonatal, juvenile, and adult testes in the common marmoset (Callithrix jacchus) to determine primate-specific patterns of gene expression that underpin primate germ cell development. Initially we characterized adult spermatogonia into two main classes; mitotically active C-KIT(+)Ki67(+) cells and mitotically quiescent SALL4(+)PLZF(+)LIN28(+)DPPA4(+) cells. We then explored the expression of a set of markers, including PIWIL1/MARWI, VASA, DAZL, CLGN, RanBPM, SYCP1 and HAPRIN, during germ cell differentiation from early spermatocytes through round and elongating spermatids, and a clear program of gene expression changes was determined as development proceeded. We then examined the juvenile marmoset testis. Markers of gonocytes demonstrated two populations; one that migrates to the basal membrane where they form the SALL4(+) or C-KIT+ spermatogonia, and another that remains in the lumen of the seminiferous tubule. This later population, historically identified as pre-spermatogonia, expressed meiotic and apoptotic markers and were eliminated because they appear to have failed to correctly migrate. Our findings provide the first platform of gene expression dynamics in adult and developing germ cells of the common marmoset. Although we have characterized a limited number of genes, these results will facilitate primate spermatogenesis research and understanding of human reproduction. (C) 2015 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available