Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 52, Issue 2, Pages 226-235Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2015.05.014
Keywords
microRNAs; Grouper; SGIV; Deep sequencing; miRNA microarray; Functional annotation
Categories
Funding
- Natural Science Foundation of China [41206137, 31330082]
- National Basic Research Program of China (973) [2012CB114402]
- Knowledge Innovation Program of Chinese Academy of Sciences [SQ201116]
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microRNAs (miRNAs) are an evolutionarily conserved class of non-coding RNA molecules that participate in various biological processes. Employment of high-throughput screening strategies greatly prompts the investigation and profiling of miRNAs in diverse species. In recent years, grouper (Epinephelus spp.) aquaculture was severely affected by iridoviral diseases. However, knowledge regarding the host immune responses to viral infection, especially the miRNA-mediated immune regulatory roles, is rather limited. In this study, by employing Solexa deep sequencing approach, we identified 116 grouper miRNAs from grouper spleen-derived cells (GS). As expected, these miRNAs shared high sequence similarity with miRNAs identified in zebrafish (Danio rerio), pufferfish (Fugu rubripes), and other higher vertebrates. In the process of Singapore grouper iridovirus (SGIV) infection, 45 and 43 miRNAs with altered expression (>1.5-fold) were identified by miRNA microarray assays in grouper spleen tissues and GS cells, respectively. Furthermore, target prediction revealed 189 putative targets of these grouper miRNAs. (C) 2015 Elsevier Ltd. All rights reserved.
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