Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 53, Issue 1, Pages 63-69Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2015.06.013
Keywords
Zebrafish; Macrophage; Infection; Cancer; Transgenic; Immunity
Categories
Funding
- Ministry of Business, Innovation and Employment, New Zealand [UOAX0813]
- New Zealand Ministry of Business, Innovation & Employment (MBIE) [UOAX0813] Funding Source: New Zealand Ministry of Business, Innovation & Employment (MBIE)
Ask authors/readers for more resources
Macrophages are the most functionally heterogenous cells of the hematopoietic system. Given many diseases are underpinned by inappropriate macrophage activation, macrophages have emerged as a therapeutic target to treat disease. A thorough understanding of what controls macrophage activation will likely reveal new pathways that can be manipulated for therapeutic benefit. Live imaging fluorescent macrophages within transgenic zebrafish larvae has provided a valuable window to investigate macrophage behavior in vivo. Here we describe the first transgenic zebrafish line that reports macrophage activation, as evidenced by induced expression of an immunoresponsive gene 1(irg1):EGFP transgene. When combined with existing reporter lines that constitutively mark macrophages, we reveal this unique transgenic line can be used to live image macrophage activation in response to the bacterial endotoxin lipopolysaccharide and xenografted human cancer cells. We anticipate the Tg(irg1:EGFP) line will provide a valuable tool to explore macrophage activation and plasticity in the context of different disease models. (C) 2015 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available