Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 323, Issue -, Pages 426-433Publisher
ELSEVIER
DOI: 10.1016/j.jhazmat.2016.03.014
Keywords
Antibiotic resistance; beta-lactams resistance gene; DNA; Water disinfection
Categories
Ask authors/readers for more resources
Water is one of the most important habitats and route for the spread of antibiotic resistance (AR) in the environment and disinfection processes can be a potential barrier to minimise this risk. In this study the effect of UV/H2O2 process on the potential of AR transfer was investigated through cultivation methods vs (polymerase chain reaction) PCR based methods. bIa(TEM) was selected as target antibiotic resistance gene (ARG) and was quantified by qPCR in the survived colonies and the whole suspension (total DNA). The detection limit of residual antibiotic resistant Escherichia coli (E. coli) colonies (5 CFUmL(-1)) was reached after 240min treatment, but b/aTEm gene was still present in total DNA after 300min (2.8 x 10(6) copies mL(-1)), and no effect was observed in DNA extracted from cell cultures (3.8 x 10(8) copies mL(-1) after 90 min). Accordingly, the investigated disinfection process may select for unaffected ARGs, therefore contributing to the potential transfer of AR in the environment. (C) 2016 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available