Journal
JOURNAL OF GENERAL VIROLOGY
Volume 98, Issue 6, Pages 1521-1525Publisher
MICROBIOLOGY SOC
DOI: 10.1099/jgv.0.000798
Keywords
TSWV; NSs; resistance breaking; Tsw gene; transient expression; complementation
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Funding
- Bolyai Janos fellowship of the Hungarian Academy of Sciences
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The nonstructural protein (NSs) of Tomato spotted wilt virus (TSWV) was previously identified as an avirulence determinant for Tsw-based resistance on pepper. The NSs of wild-type (WT) and resistance-breaking (RB) TSWV strains isolated in Hungary had only two amino acid substitutions (104, 461). We have analysed the ability of the NSs and their point mutant variants to trigger Tsw-mediated hypersensitive responses and RNA silencing suppressor (RSS) activity in patch assays. We identified a single amino acid change at position 104 (T-A) that was responsible for the necrosis induction or loss, white a significant difference was not detected in the RSS activity of the two parental strains. We have successfully complemented the infection of the WT strain on resistant pepper cultivar with the infectious S RNA transcript of the RB strain and the WT-T104A point mutant. Our work provides direct evidence that a single amino acid change can induce an RB phenotype.
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