Journal
JOURNAL OF GENERAL PLANT PATHOLOGY
Volume 83, Issue 4, Pages 231-239Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s10327-017-0721-z
Keywords
Bacterial wilt; Gold nanoparticles; Visual colorimetric detection; Genomic DNA
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Funding
- Research Council of Tarbiat Modares University, Tehran, Iran
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Ralstonia solanacearum, the devastating causal agent of potato bacterial wilt, is a soil-borne bacterium that can survive in the soil for a long time. The development of sensitive on-field detection methods for this pathogen is highly desirable due to its widespread host range and distribution. A novel nanobiosensor was thus developed to detect unamplified genomic DNA of R. solanacearum in farm soil. Gold nanoparticles functionalized with single-stranded oligonucleotides served as a probe to detect R. solanacearum genomic DNA. The advantages of this strategy include rapidity, facile usage and being a visual colorimetric method.
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