Green Synthesis of Silver Nanoparticles by Sonicated Method using Pulicha indeca Stem Extract: Characterization Antibacterial and Anticancer Activity and Cell Viability Test

In the present work, green synthesized silver nanoparticles by sonofication method using Pulicha indeca extract, which can act as reducing as well as stabilizing agent. The silver nanoparticles were characterized by UV-Vis spectra, FTIR, XRD, SEM and TEM analysis. The UV-Vis SPR peak was observed at 440 nm, which represents the characteristic plasmon resonance of nanostructures. The F.C.C crystalline structure of silver nanoparticles is evident from XRD studies. The shape and size of synthesized AgNPs were studied by TEM. The synthesized AgNPs, were mostly mono-dispersed and spherical in shape. The particles are well separated from each other and did not exhibit any aggregation. This indicates the effective capping nature of Pulchea indeca extract. The AgNPs size distribution histogram and the average size of AgNPs was found to be 14 +/- 2 nm. The effect of silver ion concentration range from 1mm to 10mm and extract concentration 1% to 8% has been studied on the formation of silver nano particles. The AgNPs shows positive antibacterial acivity against Stayphylococus aureous, Klebisella pneumonia, Bacillus subtilis and negative antibacterial activity against Protious mirabilis bacteria studies. The anticancer activity of AgNps shows positive activity on HeLa cell.