Journal
JOURNAL OF FOOD QUALITY
Volume -, Issue -, Pages -Publisher
WILEY-HINDAWI
DOI: 10.1155/2017/5239487
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Funding
- Basic Science Research Program through the National Research Foundation (NRF) of Korea - Ministry of Education [2014R1A1A2055619]
- National Research Foundation of Korea [2014R1A1A2055619] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The goal of this research is to develop a single-walled carbon nanotube-(SWCNT-) based biosensor to detect Staphylococcus aureus. The specificity of 11 bacteria and polyclonal anti-Staphylococcus aureus antibodies (pAbs) was determined using an indirect ELISA. The pAbs were immobilized onto sensor platform after the hybridization of 1-pyrenebutanoic acid succinimidyl ester (PBASE). The resistance difference (Delta R) was calculated using a potentiostat. The bacteria detected by the biosensor were observed using a scanning electron microscope (SEM). The optimum concentration of SWCNTs on the platform was determined to be 0.1mg/mL. The binding of pAbs with S. aureus resulted in a significant increase in resistance value of the biosensor (p < 0.05). The SEM images confirmed the specific binding of S. aureus on the biosensor. The SWCNT-based biosensor was able to detect S. aureus with a limit of detection (LOD) of 4 log CFU/mL.
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