Journal
JOURNAL OF ENVIRONMENTAL SCIENCES
Volume 52, Issue -, Pages 204-209Publisher
SCIENCE PRESS
DOI: 10.1016/j.jes.2016.04.018
Keywords
Autoinducer-2; Quorum sensing; Signal transduction; HPLC-MS/MS; Quantitative analysis; Escherichia coli wild type
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Funding
- National Natural Science Foundation of China [21261160489, 21207031, 51538011]
- Program for Changjiang Scholars and Innovative Research Team in University, China
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Autoinducer 2 (AI-2), an important bioactive by-product of the LuxS-catalyzed S-ribosylhomocysteine cleavage reaction in the activated-methyl-cycle, has been suggested to serve as a universal intra-and inter-species signaling molecule. The development of reliable and sensitive methods for quantitative determination of AI-2 is highly desired. However, the chemical properties of AI-2 cause difficulty in its quantitative analysis. Herein, we report a high performance liquid chromatography-tandem mass spectrometric method that enables reproducible and sensitive measurement of AI-2 concentrations in complex matrixes. 4,5-Dimethylbenzene-1,2-diamine (DMBDM), an easy-to-obtain commercial reagent, was used for the derivatization treatment. The assay was linear in the concentration range of 1.0-1000 ng/mL (R-2 = 0.999) and had a lower limit of quantification of 0.58 ng/mL. The method exhibited several advantages, e.g., high selectivity, wide linear response range, and good sensitivity. Furthermore, the effectiveness of the method was further validated through measuring AI-2 concentrations in the cell-free culture supernatant from Escherichia coli wild type. (C) 2016 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
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