Bovine serum albumin-based nanoparticles containing resveratrol: Characterization and antioxidant activity

In this study, bovine serum albumin (BSA) nanoparticles containing resveratrol (RVT) were developed to improve the biopharmaceutical characteristics of the RVT. BSA nanoparticles containing RVT (RVT-NP) were obtained by the coacervation technique and presented mean diameter of 175 +/- 6 nm, polydispersity of 0.130 +/- 0.01, zeta potential of -37.4 +/- 1.3 mV and encapsulation efficiency about 60%. Morphological analysis revealed spherical and smooth nanoparticles. In vitro release profile was prolonged and after 144 h about 26% of RVT were released. The release kinetic followed the second order model, characterized by a rapid initial release followed by a slower RVT release diffusion-controlled. Nanoencapsulation process resulted in RVT amorphization and chemical interactions between RVT and BSA. Nanoparticles were stable over 90 days at room temperature and under refrigeration (2-8 degrees C). In the hypochlorous acid scavenging activity, RVT-NP presented inferior activity than free RVT, probably due to the prolonged RVT release. Blank BSA nanoparticles also presented scavenging activity, thus, antioxidant activity of RVT-NP was an addition of the effects of the RVT and BSA. Thus, BSA nanoparticles are promising carriers for prolonged RVT release and application in oxidative stress-based diseases. (C) 2017 Elsevier B.V. All rights reserved.