Journal
JOURNAL OF DAIRY SCIENCE
Volume 100, Issue 11, Pages 8826-8837Publisher
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2017-12801
Keywords
Cronobacter; transcriptome analysis; virulence factor; flagella; outer membrane protein
Funding
- National Natural Science Foundation of China [31671951]
- Anhui Provincial Grand Project Special of Science and Technology (China) [15czz03109]
- Science and Technology Planning Project of Guangdong Province (China) [2016A050502033]
- Project of Science and Technology in Guangzhou (China) [201604020036]
- State Key Laboratory of Applied Microbiology Southern China Open Foundations, Guangzhou, China [SKLAM004-2015]
Ask authors/readers for more resources
Cronobacter species are important foodborne pathogens causing severe infections in neonates through consumption of contaminated powdered infant formula. However, the virulence-associated factors in Cronobacter are largely unknown. In this study, the transcriptome analysis between highly virulent Cronobacter sakazakii G362 and attenuated L3101 strains was used to reveal the potential factors involved in virulence. The total transcripts were grouped into 20 clusters of orthologous group categories and summarized in 3 gene ontology categories (biological process, cellular component, and molecular function). In addition, the differentially expressed genes (DEG) between these isolates were analyzed using Volcano plots and gene ontology enrichment. The predominant DEG were flagella associated genes such as flhD, motA, flgM, flgB, and fliC. Furthermore, the expression abundance of outer membrane protein or lipoprotein genes (ompW, slyB, blc, to1C, and/o/A), potential virulence-related factors (hlyIII and hha), and regulation factors (sdiA, cheY, Bss, flit) was also significantly different between G362 and L3101. Interestingly, 3 hypothetical protein genes (ESA_01022, ESA_01609, and ESA_00609) were found to be expressed only in G362. Our findings provide valuable transcriptomic information about potential virulence factor genes, which will be needed in future molecular biology studies designed to understand the pathogenic mechanism of Cronobacter. Key words: Cronobacter, transcriptome
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available