Journal
JOURNAL OF CRANIOFACIAL SURGERY
Volume 28, Issue 6, Pages 1481-1485Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/SCS.0000000000003861
Keywords
GPC3; hFOBs; osteoblast; syndromic craniosynostosis
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Funding
- National Natural Science Foundation of China [81372087]
- Foundation of Shanghai Hospital Development Center [SHDC12014113]
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Craniosynostosis is a complex disease condition, which involves premature fusion of cranial vault sutures and lacks desirable treatment. Previous studies have demonstrated decreased proliferation rate of osteoblasts and downregulated expression of glypican 3 (GPC3) in syndromic craniosynostosis patients. In this study, quantitative and qualitative analysis were utilized to assess the effect of GPC3 in human fetal osteoblastic cell line, hFOB 1.19. Lentiviral transfection efficiency with green fluorescent protein images was obtained after 72 hours. Western Blot and quantitative real-time polymerase chain reaction analysis results indicated that GPC3 was overexpressed in hFOB 1.19 cells transfected with recombinant lentivirus LV-GPC3-GFP. Cell proliferation was assessed by CCK-8 assay and cell cycle progression and apoptosis were analyzed by flow cytometric assay. Results revealed that GPC3 promoted cell viability, induced cell cycle entry into S phase, and inhibited cell apoptosis. These findings provide novel ideas in understanding the pathogenesis of craniosynostosis. It also provides novel insights in the treatment of craniosynostosis by targeting GPC3.
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