Journal
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
Volume 17, Issue 1, Pages 112-124Publisher
IVYSPRING INT PUBL
DOI: 10.7150/ijms.35133
Keywords
oral squamous carcinoma; Wnt/beta Catenin pathway; stemness; tobacco
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Funding
- AIRC Grant IG2015 [16738]
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Background: HPV-positive oral squamous cell carcinomas (OSCCs) are specific biological and clinical entities, characterized by a more favorable prognosis compared to HPV-negative OSCCs and occurring generally in non-smoking and non-drinking younger individuals. However, poor information is available on the molecular and the clinical behavior of HPV-positive oral cancers occurring in smoking/drinking subjects. Thus, this study was designed to compare, at molecular level, two OSCC cell lines, both derived from drinking and smoking individuals and differing for presence/absence of HPV infection. Methods: HPV-negative UPCI-SCC-131 and HPV16-positive UPCI-SCC-154 cell lines were compared by whole genome gene expression profiling and subsequently studied for activation of Wnt/beta Catenin signaling pathway by the expression of several Wnt-target genes, PCatenin intracellular localization, stem cell features and miRNA let-7e. Gene expression data were validated in head and neck squamous cell carcinoma (HNSCC) public datasets. Results: Gene expression analysis identified Wnt/beta Catenin pathway as the unique signaling pathway more active in HPV-negative compared to HPV-positive OSCC cells and this observation was confirmed upon evaluation of several Wnt-target genes (i.e., Cyclin D I, Cdh I, Cdkn2a, Cd44, Axing, c-Myc and TcfI). Interestingly, HPV-negative OSCC cells showed higher levels of total beta Catenin and its active form, increase of its nuclear accumulation and more prominent stem cell traits. Furthermore, miRNA let-7e was identified as potential upstream regulator responsible for the downregulation of Wnt/beta Catenin signaling cascade since its silencing in UPCI-SCC-154 cell resulted in upregulation of Wnt-target genes. Finally, the analysis of two independent gene expression public datasets of human HNSCC cell lines and tumors confirmed that Wnt/beta Catenin pathway is more active in HPV-negative compared to HPV-positive tumors derived from individuals with smoking habit. Conclusions: These data suggest that lack of HPV infection is associated with more prominent activation of Wnt/beta Catenin signaling pathway and gain of stem-like traits in tobacco-related OSCCs.
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