Journal
ANIMAL BIOLOGY
Volume 70, Issue 1, Pages 81-95Publisher
BRILL ACADEMIC PUBLISHERS
DOI: 10.1163/15707563-20191095
Keywords
Acrosome reaction; fertilization; Leptin; MAPK cascades; phosphorylation; sperm
Categories
Funding
- National Natural Science Foundation of China [31672639, 41376157, 31772517]
- Science and Technology Project of Guizhou [[2017]7003, [2018]1054]
- Youth Science and Technology Talent Growth Project of Guizhou Education Department [[2017]291]
- High Level Talent Science Research Project of Guizhou University of Engineering Science [G2017005]
- Supporting Program for Top Scientific and Technological Talents in Universities of Guizhou Province [[2017]096]
- Key Discipline of Ecology of Guizhou Province [ZDXK[2013]11]
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The role of leptin has been documented in several studies, including activated threonine phosphorylation of extracellular signal-regulated kinase (ERK1/2) in the reproduction of rodents and humans. Our previous studies have demonstrated that mitogen-activated protein kinase (MAPK) cascades ERK, P38, and c-Jun N-terminal kinase (JNK) are involved in the spermatogenesis and acrosome reaction of Eriocheir sinensis. Therefore, the aim of this study was to investigate the expression of leptin and its receptor (LepR), and the effect of leptin on MAPK cascades during calcium ionophore A23187-induced spermatozoa acrosome reaction in crabs. Successful western blotting revealed a 16 kDa band for leptin, and 120 kDa and 90 kDa bands for the obese receptor (LepR), respectively, in the tested male reproductive tissues. Both leptin and LepR were localized at the pro-acrosomal vesicle and apical cap (AC) of spermatids, suggesting their role in the subsequent acrosome reaction. Moreover, acrosome reaction can be enhanced by leptin, and this effect decreased due to the anti-LepR antibody. Afterwards, we investigated the effects of leptin on MAPK cascades. The results showed that leptin mainly activated the phosphorylation of ERK, P38 and JNK proteins in the apical cap during the acrosome reaction in crab spermatozoa. This study addresses the role of leptin on spermatozoa, and suggests that leptin may induce molecular changes associated with spermatozoa during acrosome reaction.
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