Journal
JOURNAL OF CLINICAL ONCOLOGY
Volume 35, Issue 3, Pages 352-+Publisher
AMER SOC CLINICAL ONCOLOGY
DOI: 10.1200/JCO.2016.67.5264
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Funding
- OncoMed Pharmaceuticals (Inst)
- EMD Serono (Inst)
- AstraZeneca (Inst)
- G1 Therapeutics (Inst)
- Merck (Inst)
- Pfizer (Inst)
- AVEO Pharmaceuticals (Inst)
- Eli Lilly (Inst)
- Plexxikon (Inst)
- Jiangsu Hengrui Medicine (Inst)
- Symphogen (Inst)
- Corvus Pharmaceuticals (Inst)
- TESARO (Inst)
- Bayer Healthcare Pharmaceuticals (Inst)
- Bayer Pharma AG (Inst)
- Genentech (Inst)
- Rexahn Pharmaceuticals (Inst)
- Cleave Biosciences (Inst)
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Purpose Adenoid cystic carcinomas (ACCs) represent a heterogeneous group of chemotherapy refractory tumors, with a subset demonstrating an aggressive phenotype. We investigated the molecular underpinnings of this phenotype and assessed the Notch1 pathway as a potential therapeutic target. Methods We genotyped 102 ACCs that had available pathologic and clinical data. Notch1 activation was assessed by immunohistochemistry for Notch1 intracellular domain. Luciferase reporter assays were used to confirm Notch1 target gene expression in vitro. The Notch1 inhibitor brontictuzumab was tested in patient-derived xenografts from patients with ACC and in a patient with ACC who was enrolled in a phase I study. Results NOTCH1 mutations occurred predominantly (14 of 15 patients) in the negative regulatory region and Pro-Glu-Ser-Thr-rich domains, the same two hotspots seen in T-cell acute lymphoblastic leukemias, and led to pathway activation in vitro. NOTCH1-mutant tumors demonstrated significantly higher levels of Notch1 pathway activation than wild-type tumors on the basis of Notch1 intracellular domain staining (P = .004). NOTCH1 mutations define a distinct aggressive ACC subgroup with a significantly higher likelihood of solid subtype (P < .001), advanced-stage disease at diagnosis (P = .02), higher rate of liver and bonemetastasis (P <= .02), shorter relapse-free survival (median, 13 v 34 months; P = .01), and shorter overall survival (median 30 v 122 months; P = .001) when compared with NOTCH1 wildtype tumors. Significant tumor growth inhibition with brontictuzumab was observed exclusively in the ACC patient-derived xenograft model that harbored a NOTCH1 activating mutation. Furthermore, an index patient with NOTCH1-mutant ACC had a partial response to brontictuzumab. Conclusion NOTCH1 mutations define a distinct disease phenotype characterized by solid histology, liver and bone metastasis, poor prognosis, and potential responsiveness to Notch1 inhibitors. Clinical studies targeting Notch1 in a genotype-defined ACC subgroup are warranted. (C) 2016 by American Society of Clinical Oncology.
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