Journal
BIOFABRICATION
Volume 12, Issue 2, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1758-5090/ab6413
Keywords
insitu bioprinting; handheld bioprinter; microfluidics; 3D printing; burns; skin
Funding
- NSERC Training Program Organ-on-a-Chip Engineering & Entrepreneurship
- Austrian Society for Surgeons Travel Grant
- NSERC DC
- Medicine by Design
- CIHR [123336]
- NIH RO1 [2R01GM08728505A1]
- CFI Leader's Opportunity Fund [25407]
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The current standard of care for patients with severe large-area burns consists of autologous skin grafting or acellular dermal substitutes. While emerging options to accelerate wound healing involve treatment with allogeneic or autologous cells, delivering cells to clinically relevant wound topologies, orientations, and sizes remains a challenge. Here, we report the one-step in situ formation of cell-containing biomaterial sheets using a handheld instrument that accommodates the topography of the wound. In an approach that maintained cell viability and proliferation, we demonstrated conformal delivery to surfaces that were inclined up to 45 degrees with respect to the horizontal. In porcine pre-clinical models of full-thickness burn, we delivered mesenchymal stem/stromal cell-containing fibrin sheets directly to the wound bed, improving re-epithelialization, dermal cell repopulation, and neovascularization, indicating that this device could be introduced in a clinical setting improving dermal and epidermal regeneration.
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