Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 55, Issue 4, Pages 1074-1079Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.02107-16
Keywords
rapid diagnostic test; 16S rRNA methylases; antibiotic; susceptibility testing; gentamicin; amikacin; plazomicin; tobramycin; netilmicin; kanamycin
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Funding
- University of Fribourg, Switzerland
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The rapid aminoglycoside NP (Nordmann/Poirel) test was developed to rapidly identify multiple aminoglycoside (AG) resistance in Enterobacteriaceae. It is based on the detection of the glucose metabolism related to enterobacterial growth in the presence of a defined concentration of amikacin plus gentamicin. Formation of acid metabolites was evidenced by a color change (orange to yellow) of the red phenol pH indicator. The rapid aminoglycoside NP test was evaluated by using bacterial colonies of 18 AG-resistant isolates producing 16S rRNA methylases, 20 AG resistant isolates expressing AG-modifying enzymes (acetyl-, adenyl-, and phosphotransferases), and 10 isolates susceptible to AG. Its sensitivity and specificity were 100% and 97%, respectively, compared to the broth dilution method, which was taken as the gold standard for determining aminoglycoside resistance. The test is inexpensive, rapid (< 2 h), and implementable worldwide.
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