Journal
JOURNAL OF CLINICAL LABORATORY ANALYSIS
Volume 32, Issue 4, Pages -Publisher
WILEY
DOI: 10.1002/jcla.22323
Keywords
loop-mediated isothermal amplification; New Delhi metallo-b-lactamase 1
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Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico
- Fundacao de Amparo a Pesquisa do Estado de Minas Gerais
- Fundacao de Amparo a Pesquisa e Desenvolvimento Cientifico do Maranhao
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior
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Background: New Delhi Metallo-b-lactamase (NDM-1) is an enzyme emerging around the world conferring resistance to a wide range of beta-lactams agents and whose early detection is extremely important. We proposed to standardize the detection of the bla(NDM-1) gene using the LOOP-mediated isothermal amplification technique (LAMP). Methods: In all, 14 Gram-negative bacterial strains isolated from patients presenting pneumonia associated with mechanical ventilation were used for the bla(NDM-1) standardization by LAMP. Klebsiella pneumoniae ATCC BAA-2473 and two clinical strains were used as a positive control. All results were compared to the reaction in polymerase chain reaction (PCR), considered gold standard for this detection. Results: There was an excellent correlation between the two techniques employed, since all measured clinical strains were negative in both employed tests and two clinical, and a reference strains were positive. Conclusions: The lamp technique seems to be an excellent option for the rapid detection of bla(NDM-1). The amplification time is much shorter than other molecular techniques, the PCR machine is not necessary, it is easy of implementation and costs is low.
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