Journal
JOURNAL OF CLINICAL INVESTIGATION
Volume 127, Issue 10, Pages 3717-3729Publisher
AMER SOC CLINICAL INVESTIGATION INC
DOI: 10.1172/JCI94130
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Funding
- Italian Telethon Foundation [TCP12008]
- TIGEM
- Marie Curie [PCIG13-GA-2013-618805]
- STAR/Banco di Napoli
- Italian Ministry of Research [FIRB RBFR13LH4X]
- European Research Council [714551, 694282]
- Penn Orphan Disease Center [MPSI-16-004-01]
- National MPS Society
- NIH [U19AI199725]
- U.S. National Institutes of Health [R01-NS078072]
- European Research Council (ERC) [714551] Funding Source: European Research Council (ERC)
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The mammalian target of rapamycin complex 1 (mTORC1) kinase promotes cell growth by activating biosynthetic pathways and suppressing catabolic pathways, particularly that of macroautophagy. A prerequisite for mTORC1 activation is its translocation to the lysosomal surface. Deregulation of mTORC1 has been associated with the pathogenesis of several diseases, but its role in skeletal disorders is largely unknown. Here, we show that enhanced mTORC1 signaling arrests bone growth in lysosomal storage disorders (LSDs). We found that lysosomal dysfunction induces a constitutive lysosomal association and consequent activation of mTORC1 in chondrocytes, the cells devoted to bone elongation. mTORC1 hyperphosphorylates the protein UV radiation resistance-associated gene (UVRAG), reducing the activity of the associated Beclin 1-Vps34 complex and thereby inhibiting phosphoinositide production. Limiting phosphoinositide production leads to a blockage of the autophagy flux in LSD chondrocytes. As a consequence, LSD chondrocytes fail to properly secrete collagens, the main components of the cartilage extracellular matrix. In mouse models of LSD, normalization of mTORC1 signaling or stimulation of the Beclin 1-Vps34-UVRAG complex rescued the autophagy flux, restored collagen levels in cartilage, and ameliorated the bone phenotype. Taken together, these data unveil a role for mTORC1 and autophagy in the pathogenesis of skeletal disorders and suggest potential therapeutic approaches for the treatment of LSDs.
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