Journal
GENOME BIOLOGY
Volume 21, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s13059-020-01957-w
Keywords
Enhancer; H3; 3; Gene transcription
Funding
- Chinese Ministry of Science and Technology [2016YFA0100400, 2018YFE0203300]
- Chinese Academy of Sciences [XDB 39000000]
- China Natural Science Foundation [31530037, 31425013, 31521002]
- Youth Innovation Promotion Association of the Chinese Academy of Sciences [2017133, 2020097]
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H3K27ac is well recognized as a marker for active enhancers and a great indicator of enhancer activity. However, its functional impact on transcription has not been characterized. By substituting lysine 27 in histone variant H3.3 with arginine in mouse embryonic stem cells, we diminish the vast majority of H3K27ac at enhancers. However, the transcriptome is largely undisturbed in these mutant cells, likely because the other enhancer features remain largely unchanged, including chromatin accessibility, H3K4me1, and histone acetylation at other lysine residues. Our results clearly reveal that H3K27ac alone is not capable of functionally determining enhancer activity.
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