Journal
DENTAL MATERIALS
Volume 31, Issue 2, Pages 195-203Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dental.2014.12.004
Keywords
Cytotoxicity; Dental composites; Bioactive glass; Degree of Conversion; FTIR; Undifferentiated pulp cells (OD-21)
Funding
- NIH/NIDCR [1R01 DE021372]
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Objective. To determine the in vitro cytotoxicity of dental composites containing bioactive glass fillers. Methods. Dental composites (50:50 Bis-GMA/TEGDMA resin: 72.5 wt% filler, 67.5% Sr-glass and 5% OX50) containing different concentrations (0, 5, 10 and 15 wt%) of two sol-gel bioactive glasses, BAG65 (65 mole% SiO2, 31 mole% CaO, 4 mole% P2O5) and BAG61 (3 mole% F added) were evaluated for cytotoxicity using Alamar Blue assay. First, composite extracts were obtained from 7 day incubations of composites in cell culture medium at 37 degrees C. Undifferentiated pulp cells (OD-21) were exposed to dilutions of the original extracts for 3, 5, and 7 days. Then freshly cured composite disks were incubated with OD-21 cells (n = 5) for 2 days. Subsequently, fresh composite disks were incubated in culture medium at 37 degrees C for 7 days, and then the extracted disks were incubated with OD-21 cells for 2 days. Finally, fresh composites disks were light cured for 3, 5, and 20 s and incubated with OD-21 cells (n = 5) for 1,3, 5, and 7 days. To verify that the three different curing modes produced different levels of degree of conversion (DC), the DC of each composite was determined by FTIR. Groups (n = 5) were compared with ANOVA/Tukey's (alpha <= 0.05). Results. Extracts from all composites significantly reduced cell viability until a dilution of 1:8 or lower, where the extract became equal to the control. All freshly-cured composites showed significantly reduced cell viability at two days. However, no reduction in cell viability was observed for any composite that had been previously soaked in media before exposure to the cells. Composites with reduced DC (3 s vs. 20 s cure), as verified by FTIR, showed significantly reduced cell viability. Significance. The results show that the composites, independent of composition, had equivalent potency in terms of reducing the viability of the cells in culture. Soaking the composites for 7 days before exposing them to the cells suggested that the toxic components had been extracted and the materials were no longer cytotoxic. The results demonstrate that the cytotoxicity of composites with and without BAG must predominantly be attributed to the release of residual monomers, and not to the presence of the BAG. (C) 2014 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
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