Journal
FRONTIERS IN SYNAPTIC NEUROSCIENCE
Volume 12, Issue -, Pages -Publisher
FRONTIERS RESEARCH FOUNDATION
DOI: 10.3389/fnsyn.2020.00006
Keywords
rabies; virus; monosynaptic tracing; AAV (adeno-associated virus); circuit tracing
Categories
Funding
- BRAIN Initiative [U01MH106018, U01MH114829, U19MH114830]
- National Institute of Mental Health
Ask authors/readers for more resources
Monosynaptically-restricted transsynaptic tracing using deletion-mutant rabies virus (RV) has become a widely used technique in neuroscience, allowing identification, imaging, and manipulation of neurons directly presynaptic to a starting neuronal population. Its most common implementation is to use Cre mouse lines in combination with Cre-dependent helper adeno-associated viral vectors (AAVs) to supply the required genes to the targeted population before subsequent injection of a first-generation (DG) rabies viral vector. Here we show that the efficiency of transsynaptic spread and the degree of nonspecific labeling in wild-type control animals depend strongly on the concentrations of these helper AAVs. Our results suggest practical guidelines for achieving good results.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available