Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1060, Issue -, Pages 150-157Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2017.05.036
Keywords
Pummelo peel; Limonin; Water extraction; Ammonium sulfate precipitation; Purification; Identification
Funding
- National Natural Science Fund Project of China [31271914]
- Fund for Innovative Research Team of Jimei University [2010A006]
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Limonin is a bioactive compound that is traditionally extracted from citrus seeds using organic solvents or alkaline/metal ion solutions. In the present study, pummelo [Citrus grandis] peel was investigated for limonin preparation using a novel process consisting of water extraction, ammonium sulfate precipitation and resin adsorption. The pummelo peel was determined to have 4.7 mg/g limonin, which could be extracted by water and further recovered by ammonium sulfate precipitation with a yield of 2.4 mg/g, which was similar to that of traditional process using ethanol extraction and vacuumed evaporation. The precipitated limonin was purified by resin adsorption and crystallization with a purity of 96.4%. In addition, the limonin was identified via the analyses of retention time, infrared spectrum and nuclear magnetic resonance. This study indicates a novel and eco-friendly process for recovering limonin, providing a new candidate for limonin preparation.
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