Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1061, Issue -, Pages 364-371Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2017.08.001
Keywords
Essential oil; Modified-solvent free microwave extraction; Amomun tsao-ko; Antioxidant activity; Antimicrobial activity; Oxygenated monoterpenes
Funding
- National Key R & D Program of China [2016YFD0600805]
- Fundamental Research Funds for the Central Universities [2572017EA03, 2572017DA04, 2572015EA04, 2572016AA48]
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A simple, green and efficient extraction method named modified-solvent free microwave extraction (M-SFME) was employed for the extraction of essential oils (EOs) from Amomun tsao-ko. The process of M-SFME was optimized with the prominent preponderance of such higher extraction yield (1.13%) than those of solvent free microwave extraction (SFME, 0.91%) and hydrodistillation (HD, 0.84%) under the optimal parameters. Thirty-four volatile substances representing 95.4% were identified. The IC50 values of EOs determined by DPPH radical scavenging activity and beta-carotene/linoleic acid bleaching assay were 5.27 and 0.63 mg/ml. Furthermore, the EOs exhibited moderate to potent broad-spectrum antimicrobial activity against all tested strains including five gram-positive and two gram-negative bacteria (MIC: 2.94-5.86 mg/ml). In general, M-SFME is a potential and desirable alternative for the extraction of EOs from aromatic herbs, and the EOs obtained from A. tsao-ko can be explored as a potent natural antimicrobial and antioxidant preservative ingredient in food industry from the technological and economical points of view.
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