Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1053, Issue -, Pages 1-8Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2017.03.035
Keywords
Thymus schimperi; Polyphenols; Isolation; Tandem mass spectrometry; Anti-proliferative effects; Cancer cells
Funding
- National Research Foundation (NRF) of Korea - Ministry of Science, ICT and Future Planning [2012M3A9B8019303, 2017R1A2B4003974]
- National Research Foundation of Korea [2017R1A2B4003974] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Thymus schimperi is a highly localized and a rare plant endemic to Ethiopia. An optimized and validated high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method was applied to characterize 23 polyphenolic compounds found in ethyl acetate extracts of the plant. From those, flavones dominated and luteolin was the major component contributing 21.83% of the total composition (or 46.05 +/- 0.59 g/kg of fresh sample weight). Validation data showed a determination coefficient (R-2) >= 0.997. Limits of detection (LOD) and quantification (LOQ) were 0.03-0.97 and 0.11-3.23 mg/L, while recovery values spiked at 5 and 50 mg/L were between 70.89-115.39 and 67.65-120.19%, respectively. Except for caffeic acid and epicatechin gallate, the relative standard deviations (%RSD5) were far below 15%, showing acceptable precision values. The plant extracts inhibited cell proliferation and induced cell death in human gastric adenocarcinoma (AGS) and liver hepatocellular carcinoma (HepG2) cancer cells. This is the first report of polyphenolic components from T. schimperi being characterized using HPLC-ESI-MS/MS. Being components of many edible vegetables, fruits, and spices, the identified polyphenols suggest that T. schimperi could be a potential food with promising health benefits. (C) 2017 Elsevier B.V. All rights reserved.
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