Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1053, Issue -, Pages 27-33Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2017.04.006
Keywords
Veterinary drugs; Residues analysis; Animal matrices; Multi-plug filtration cleanup
Funding
- National Instrumentation Program of China [2013YQ510391]
- Chinese Ministry of Science and Technology
- International Atomic Energy Agency (IAEA) [NAM/14003]
- program for Guangxi Special Invited Scientist in Agric-Environment and Agroproducts Safety
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The frequent use of various veterinary drugs could lead to residue bioaccumulation in animal tissues, which could cause dietary risks to human health. In order to quickly analyze the residues, a liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for detecting Sulfonamides, Tilmicosin and Avermectins (AVMs) residues in animal samples. For sample preparation, modified QuEChERS (quick, easy, cheap, effective, rugged and safe) and ultrasound-assisted extraction (UAE) methods were used. For sample cleanup, n-Hexane delipidation and multi-plug filtration cleanup (m-PFC) method based on primary-secondary amine (PSA) and octadecyl-silica (C18) were used, followed by LC-MS/MS analysis. It was validated on 7 animal matrices (bovine, caprine, swine meat and their kidneys, milk) at two fortified concentration levels of 5 and 100 mu g/kg. The recoveries ranged from 82 to 107% for all analytes with relative standard deviations (RSDs) less than 15%. Matrix-matched calibrations were performed with coefficients of determination above 0.998 for all analytes within concentration levels of 5-500 mu g/kg. The developed method was successfully used to analysis veterinary drugs of real animal samples from local markets.
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