Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1051, Issue -, Pages 24-32Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2017.02.022
Keywords
Ripening; Anacardic acid; Phenolics; Cashew apple; Enzymes
Funding
- Institute Nacional de Ciencia e Tecnologia de Frutos Tropicais - INCT/CNPq, Brazil
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-CAPES, Brazil
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Cashew immature and ripe peduncles (Anacardium occidentale L) from orange- and red-colored clones CCP 76 and BRS 189, respectively, were prepared as juice or fibrous fraction and submitted to UPLC-MS analyses, while the soluble fraction was also submitted to enzymatic evaluation. Cinnamoyl glucoside was present in ripe juice samples from both cashew clones, while monogalloyl diglucoside and digalloyl glucoside were present in immature juice samples from both cashew clones. Four compounds were found at immature fiber of both clones, anacardic acids (1, 2, 3) and GA(19). The phenolic biosynthetic pathway was evaluated in juice samples and phenylalanine ammonia-lyase activity decreased significantly during the development, although it was much higher in ripe CCP 76. UDP-glycosyltransferases activity differed between clones, however its product cinnamoyl glucoside was a possible chemical marker of ripe juice samples from both clones. Flavonol synthase showed the highest specific activity in both cashew clones and its product, fiavonols were identified in cashew apple at immature and ripe stages. (C) 2017 Elsevier B.V. All rights reserved.
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