Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1048, Issue -, Pages 111-120Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2017.02.009
Keywords
Flowers of Pueraria lobata; Stroke; Lactate dehydrogenase inhibitors; Microwave-assisted extraction; Countercurrent chromatography
Funding
- National Natural Science Foundation of China [31670358, 31370374, 31500279]
- Natural Science Foundation of Jilin Province [20150520140JH]
- China Postdoctoral Science Foundation [2016M590267]
- Natural Science Foundation of Changchun Normal University [[2015]008, [2015]009]
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A simple and efficient method based on ultrafiltration liquid chromatography-mass spectrometry (UFLC-MS) was applied to rapidly screen and identify ligands for lactate dehydrogenase (LDH) from the flowers of Pueraria lobata, and the compounds were assessed for anti-stroke activity using a PC12 cell model. Seven major isoflavones, kakkalide, 3'-hydroxy puerarin, puerarin, puerarin xyloside, tectoridin, tectorigenin, and ononin, were identified as potent LDH inhibitors. A continuous online method, which consisted of microwave-assisted extraction and countercurrent chromatography (MAE-CCC), was newly developed for scaled-up production of these compounds with high purity and efficiency. This novel approach, using UFLC-MS coupled with MAE-CCC and a PC12 cell model, provided a powerful tool for screening, extraction, and separation of LDH inhibitors from complex samples, and a useful platform for the large-scale production of functional food and nutraceutical ingredients. (C) 2017 Elsevier B.V. All rights reserved.
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