Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1057, Issue -, Pages 46-53Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2017.04.040
Keywords
Alkaloids; LC MS/MS; Pharmacokinetics; Rabbit aqueous humor; Rhizoma Corydalis Decumbentis
Funding
- Key Project for Drug Innovation grant from Ministry of Science and Technology of China [2013zx09301305]
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This study aims to establish a fast and sensitive LC-MS/MS method for simultaneous determination of seven alkaloids from Rhizome Corydalis Decumbentis in rabbit aqueous humor. Aqueous humor samples were processed by protein precipitation and then separated on a Thermo Syncronis Cut column (50 mm x 2.1 mm, 5 mu m) with a mobile phase using acetonitrile-0.05% formic acid (28:72, v/v). Detection of the analytes and the internal standard (coptisine) were performed in positive electrospray ionization with selected reaction monitoring. The method showed good linearity (r > 0.9931) for all the seven alkaloids. This fully validated method was applied to the studies of aqueous humor pharmacokinetics of seven alkaloids from Rhizome Corydalis Decumbentis and the effects of bomeol on corneal penetration of these alkaloids into aqueous humor. This is the first work that presents a reliable LC-MS/MS method for simultaneous determination of seven alkaloids in rabbit aqueous humor and its application of ocular pharmacokinetics of seven alkaloids from Rhizome Corydalis Decumbentis.
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