Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1040, Issue -, Pages 67-72Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2016.11.018
Keywords
Sulfation; Sulfotransferase; HILIC; Phenylephrine; Drug metabolism
Funding
- Pfizer Consumer Healthcare through Investigator Initiated Research Program
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The efficacy of phenylephrine (PE) is controversial due to its extensive pre-systemic metabolism through sulfation to form phenylephrine-3-O-sulfate (PES). Hence quantitation of PES is important in order to study the metabolism of PE. There are no published methods available for direction detection of PES. We have developed and validated a hydrophilic interaction liquid chromatography (HILIC) method for the direct detection of PES and simultaneous detection of PE to study the enzyme kinetics and metabolism of PE to enable approaches to reduce the presystemic metabolism of PE. This is the first method which facilitates direct detection of PES and simultaneous detection of PE using a zwitterionic HILIC column with improved sensitivity in a single short run. The observed quantitative ranges of our method for PE and PES were 0.39-200 mu M and 0.0625-32 mu M (respectively) with a run time of 6.0 min. The method was applied to the determination of PE and PES in LS180 human intestinal cell line, recombinant enzymes and human intestinal cytosol (HIC). (C) 2016 Elsevier B.V. All rights reserved.
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