Solvent Front Position Extraction procedure with thin-layer chromatography as a mode of multicomponent sample preparation for quantitative analysis by instrumental technique

A concept of using thin-layer chromatography to multicomponent sample preparation for quantitative determination of solutes followed by instrumental technique is presented. Thin-layer chromatography (TLC) is used to separate chosen substances and their internal standard from other components (matrix) and to form a single spot/zone containing them at the solvent front position. The location of the analytes and internal standard in the solvent front zone allows their easy extraction followed by quantitation by HPLC. (C) 2017 Elsevier B.V. All rights reserved.