Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1512, Issue -, Pages 88-97Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2017.07.032
Keywords
Glycosylation monolith; Polymer monolithic microextraction; Glycocluster grafted beta-cyclodextrin; Glycopeptides; Monolithic hybrid polymer
Funding
- National Natural Science Foundation of China [21575049]
- Open Project of State Key Laboratory of Supramolecular Structure and Materials, Jilin University [skissm201617]
Ask authors/readers for more resources
The low abundance of glycoproteins in complex samples results in the prerequisite role of efficient and selective enrichment of them. In the present work, we designed a new kind of glycosylation poly(hydroxyethyl methacrylate-pentaerythritol triacrylate) monolith functionalized with glycocluster grafted p-cyclodextrin for the enrichment of glycoproteins. The introduced modifiers endowed the monolithic material with enhanced hydrophilicity and surface area, which benefitted to improve the enrichment selectivity and extraction efficiency for glycopeptides. By combining with MALDI-MS detections, 22 glycopeptides from horseradish peroxidase digest were captured with the developed monolith while 4 glycopeptides were enriched by commercially available agarose matrix column. LOD of 6.6 pmol was attained. When applied to the enrichment of glycopeptides from complex protein samples and human lymphoma (U937) cell line, the prepared monolith exhibited high selectivity for glycopeptides. (C) 2017 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available